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Séminaire MIO : Anaïs Cario (Post Doc) de l’Institut de Chimie de la Matière Condensée de Bordeaux : Can we use Microfluidics for investigating the Deep Biosphere ?

Version imprimable de cet article

Le 17 janvier 2019 à 13h dans l’amphi du bâtiment Méditerranée

Can we use Microfluidics for investigating the Deep Biosphere ?

Anaïs Cario*, Olivier Nguyen, Carole Lecoutre, Yves Garrabos, Samuel Marre*
CNRS, Univ. Bordeaux, ICMCB, F-33600, Pessac Cedex, France

Key words : Microfluidic, High Pressure, Deep Biosphere

Abstract :

A majority of Earth’s prokaryotes reside in the deep biosphere where little is known about how inherent elevated pressures impact the underground geochemistry and the inhabiting microbial communities. Conventional cultivation and analysis techniques offer both limited optical access and in situ characterization, thus narrowing the ability to investigate deep subsurface microbial communities. High pressure microfluidic approaches offer new opportunities to study and characterize high pressure fluid processes (up to 300 bar). These approaches combine the microfluidic advantages (i.e. hydrodynamic control, fast screening, small volumes, in situ and online characterization, etc.) to fluid properties under high pressure and temperature conditions. Besides, microfluidic tools allow performing studies at the pore scale and are complementary from classical high pressure methods (i.e. batch reactors and anvil cells).
This presentation will first highlight the basic knowledge for HP microfabrication. Then we will introduce the use of Bio-Geological Laboratories on a Chip (BioGLoCs) as significant tools to mimic the in situ biogeological reservoirs conditions to study CO2 bioconversion within deep aquifers (in the frame of the ERC project “Big Mac”). In this context, we will also present some ongoing work aiming at integrating in situ spectroscopy techniques in HP microreactors to get information about the dissolution and mineralization trapping.
Eventually, we will emphasize on the possible use of these tools to wider applications in geological-related applications, but also on deep-sea field studies.